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The organic phases were combined, washed twice with saturated brine, dried over anhydrous sodium sulfate, and purified to obtain a white solid product S1 (240.4 mg, purity 92%)

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Interestingly, introduction of carboxylic acid groups to RT slows down its reaction kinetics towards GSH comparing to RT-NH 2 and TQG-CN (Table 2), presumably due to the charge repulsion between the carboxylic acid groups and the anionic GSH